Abstract:
Mycoplasma bovis is a major pathogen, responsible for bovine respiratory diseases worldwide. The present lack of effective control measures leaves cattle owners at considerable perpetual risk of M. bovis outbreaks. In this study, we identified M. bovis secreted immunogenic proteins in silico as potential candidates for novel diagnostic agents and vaccines. We used immunoinformatics to analyze 438 M. bovis proteins previously identified with a label-free proteomics analysis of virulent M. bovis HB0801 (P1) and its attenuated P150 strains. The subcellular localization of these proteins was preliminarily screened and 59 proteins were found to be secreted extracellular proteins. Twenty-seven of these proteins contained a large number of predictive T-cell epitopes presented by major histocompatibility complex (MHC) class I and II molecules. Twenty-two of these 27 proteins had a high number of conformational B-cell epitopes, predicted from the corresponding 3D structural templates, including one unique to P1, two unique to P150, and 19 common to both strains. Five proteins were selected for further validation, and two of these, MbovP274 and MbovP570, were successfully expressed and purified. Both were confirmed to be secretory and highly immunogenic proteins that induced a mouse antibody response, reacted with cattle serum positive for M. bovis infection, and significantly increased the production of interleukin 8 (IL-8), IL-12 and interferon γ(IFN-γ) during the secretion of these three cytokines by both M. bovis mutants of these genes. These results should be useful in the development of novel immunological agents against M. bovis infection.
Keywords: Mycoplasma bovis; secretory proteins; immunoinformatics; antigenicity; epitopes; inflammatory response.
Biography:
Education Background
M.V.Sc. 20017-2020, majoring in Preventive veterinary Medicine at College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Bachelor degree 2007-2012, at College of Veterinary Medicine and Animal Science, Nangarhar University, Jalalabad, Afghanistan.
Experience
Sep. 2012-present, working as Lecture and Assistant Professor in animal infectious diseases and public health, advisor for graduate students for bachelor in College of Veterinary Medicine, Nangarhar University, Afghanistan. Member of research committee of Veterinary Science Faculty, Nangarhar University, Afghanistan.
Areas of Research Interests/ Research
His current research interested in basic and applied research on bovine infectious diseases and zoonosis, Molecular biology. The main areas are involved in epidemiology and etiology, pathogenesis and immunology, development of novel vaccines and diagnostic techniques. He currently focus on the pathogens responsible for respiratory and gastrointestinal diseases such as Mycobacterium bovis, Mycoplasma bovis, etc.
Academic Performance
He is engaged in the basic and applied research related to the prevention and control of animal tuberculosis and bovine infectious diseases. In the past three years, published 3 papers, including one SCI papers.
